Evaluation of Potential DNA Sources and Need of DNA Analysis for Personalized Medicine in Microfluidic Device

Abstract

DNA analysis is one of primary requirement in genetic studies for disease diagnosis, treatment and precaution steps as the characterization of many diseases are linked with genes. Particularly, genetic testing is vital to detect heritable disease or developing infectious disease, and newborn screening to identify genetic disorder that can be treated at early stage. Fundamentally the raw source of DNA such as blood, saliva, tissue section and urine consist of complex matrices with heterogeneous contents caused traditional bio-analytical methods difficult to perform plus time consuming analysis. Thus, DNA extraction from most appropriate biological sample for downstream process to replace conventional method has been widely studied due to its crucial role in clinical diagnostic and genetic analysis. Meanwhile, the growing technology has recognized the importance of microfluidic platform as healthcare point of device especially in addressing the issue needs for modern biological rapid synthesis and analysis by using only small amount of sample and reagent volumes. This paper highlighted the potential DNA sources that can be used for DNA analysis in microfluidic point of care device.